KLK7 ELISA Kit (Mouse) (OKCD02671)

Catalog No: OKCD02671

Size:96WELLS

Mouse KLK7 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-105%	82-101%	92-99%	88-95%
  EDTA Plasma (n=5)	80-91%	93-101%	83-101%	86-104%
  Heparin Plasma (n=5)	91-99%	82-101%	80-101%	89-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 7 (KLK7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 7 (KLK7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 7 (KLK7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kallikrein 7 (KLK7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-101	83
  EDTA Plasma (n=5)	80-105	84
  Heparin Plasma(n=5)	91-99	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 7 (KLK7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 7 (KLK7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KLK7 Microplate: 96 Wells (12 x 8 Well strips)
  • KLK7 Lyophilized Standard: 2
  • 100X Biotinylated KLK7 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 32 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kallikrein 7 (KLK7). No significant cross-reactivity or interference between Kallikrein 7 (KLK7) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Klk7
• Gene Full Name: kallikrein related-peptidase 7 (chymotryptic, stratum corneum)
• Alias Symbols: kallikrein 7 (chymotryptic, stratum corneum);kallikrein-7;P;Prss6;SCCE;serine protease 6;stratum corneum chymotryptic enzyme;thymopsin.
• NCBI Gene Id: 23993
• Protein Name: Kallikrein-7
• Description of Target: May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.
• Uniprot ID: Q91VE3
• Protein Accession #: NP_036002.1
• Nucleotide Accession #: NM_011872.3
• Protein Size (# AA): 249