Catalog No: OKCD00271 (Formerly GWB-KBBMJ3)
Size:96WELLS
Price: $650.00
SKU
OKCD00271
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Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kruppel Like Factor 4, Gut (KLF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kruppel Like Factor 4, Gut (KLF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kruppel Like Factor 4, Gut (KLF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kruppel Like Factor 4, Gut (KLF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kruppel Like Factor 4, Gut (KLF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kruppel Like Factor 4, Gut (KLF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-KLF4 Microplate96 Wells (12 x 8 Well Strips)
KLF4 Lyophilized Standard2 x 10 ng
100X Biotinylated KLF4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Kruppel Like Factor 4, Gut (KLF4). No significant cross-reactivity or interference between Kruppel Like Factor 4, Gut (KLF4) and analogues was observed.
Publications

KLF4 Promotes Angiogenesis by Activating VEGF Signaling in Human Retinal Microvascular Endothelial Cells. PLoS One. 10, e0130341 (2015). 26075898

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Description
Gene SymbolKLF4
Gene Full NameKruppel like factor 4
Alias Symbolsendothelial Kruppel-like zinc finger protein;epithelial zinc finger protein EZF;EZF;GKLF;gut Kruppel-like factor;gut-enriched krueppel-like factor;Krueppel-like factor 4;Kruppel-like factor 4 (gut).
NCBI Gene Id9314
Protein NameKrueppel-like factor 4
Description of TargetTranscription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Uniprot IDO43474
Protein Accession #NP_001300981
Nucleotide Accession #NM_001314052.1
Protein Size (# AA)513
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