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Now Offering Over 85,203 Antibodies & 34,301 Antigens!

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50ug
$289.00
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KLF2 antibody - middle region (ARP32760_P050)

Receive a free positive control (AHL008) when you purchase this antibody. Use the promotion code 'freecontrol' when placing your order.
Please go here for more details.
Description of Target:
KLF2 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 3 C2H2-type zinc fingers. KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.
Gene Symbol:
KLF2
Official Gene Full Name:
Kruppel-like factor 2 (lung)
NCBI Gene Id:
10365
Alias Symbols:
LKLF
Tissue Tool:
Find tissues and cell lines supported to express KLF2.
Protein Accession #:
NP_057354
Nucleotide Accession#:
NM_016270
Swissprot Id:
Q9Y5W3
Host:
Rabbit
Clonality:
Polyclonal
Protein Name:
Krueppel-like factor 2
Protein Size (# AA):
355
Molecular Weight:
37kDa
Application:
CHIP, IHC, WB
Partner Proteins:
GEMIN4, ZFHX3, AR, ATF7IP, EP300, ESR1, ESR2, GEMIN4, GFI1, HABP4, HDAC1, HMGA2, MITF, NCOA2, NR3C2, OPN1LW, PGR, PRPF40A, RELA, SERBP1, SKIL, SMAD2, SMAD3, SMAD4, STAT3, TBP, ZFHX3, AR, CREBBP, EP300, GFI1, HMGA2, MITF, RELA, SIAH1, SIAH2, SMAD2, SMAD3, SMAD4, STAT3, TRIM32, UBC, ZMIZ1, ZMIZ2
Immunogen:
The immunogen for anti-KLF2 antibody: synthetic peptide directed towards the middle region of human KLF2
Product Format:
Lyophilized powder
Purification:
Affinity Purified
Complete computational species homology data:
KLF2 antibody - middle region (ARP32760_P050)
Predicted Homology Based on Immunogen Sequence:
Pig: 100%; Human: 100%; Rat: 93%; Mouse: 93%; Guinea pig: 93%; Zebrafish: 79%
Species Reactivity:
Pig, Human, Guinea pig, Mouse, Rat, Zebrafish
Datasheets / Downloads:
Printable datasheet for
anti-KLF2 antibody
- ARP32760_P050
Peptide Sequence:
Synthetic peptide located within the following region: PPAFGLFDDAAAAAAALGLAPPAARGLLTPPASPLELLEAKPKRGRRSWP
Blocking Peptide:
For anti-KLF2 antibody is Catalog # AAP32760 (Previous Catalog # AAPP03775)
Additional Information:
IHC Information: Brain, cerebellum
IHC Information: Colon, myenteric plexus
IHC Information: Lung
Target Reference:
Saavedra,M.T., (2008) Am. J. Respir. Cell Mol. Biol. 38 (6), 679-688
Reconstitution and Storage:
Add 50 ul of distilled water. Final anti-KLF2 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20C. Avoid repeat freeze-thaw cycles.
Publications:

Anti-KLF2 ARP32760_P050 has recently been referenced in the following publications:

Yamada, T., Park, C. S., Mamonkin, M. & Lacorazza, H. D. Transcription factor ELF4 controls the proliferation and homing of CD8+ T cells via the Krüppel-like factors KLF4 and KLF2. Nat. Immunol. 10, 618–26 (2009). 19412182

Computational species homology for KLF2 antibody (ARP32760)

Product page for KLF2 antibody (ARP32760)

The information below lists the predicted species and target name associated to the peptide antigen. Please note, all available target reference numbers to the antigen sequence are presented, including unreviewed and protein isoforms.
To search for antibodies by species, please visit Aviva’s Species Reactivity Page. To search Aviva’s catalog of antibodies by sequence, please visit Aviva’s Antibody Blast Tool.

Predicted Species & Target Target Reference Predicted Homology
Atlantic salmon klf2 antibody; Salmo salar klf2 antibody B5X170 85%
Chicken KLF2 antibody; Gallus gallus KLF2 antibody E1C442 78%
Common turkey KLF2 antibody; Meleagris gallopavo KLF2 antibody G1N228 78%
Duckbill platypus KLF2 antibody; Ornithorhynchus anatinus KLF2 antibody F7C422 92%
Gray short-tailed opossum KLF2 antibody; Monodelphis domestica KLF2 antibody F7G649 92%
Gray short-tailed opossum KLF2 antibody; Monodelphis domestica KLF2 antibody F6R7C8 92%
Guinea pig KLF2 antibody; Cavia porcellus KLF2 antibody H0W7M7 92%
Human KLF2 antibody; Homo sapiens KLF2 antibody Q9Y5W3 100%
Mouse KLF2 antibody; Mus musculus KLF2 antibody Q60843 92%
Mouse Klf2 antibody; Mus musculus Klf2 antibody Q9JLV7 92%
Mouse Klf2 antibody; Mus musculus Klf2 antibody Q3V293 92%
Northern white-cheeked gibbon KLF2 antibody; Nomascus leucogenys KLF2 antibody G1R093 100%
Pig KLF2 antibody; Sus scrofa KLF2 antibody B4X5Z2 100%
Rat KLF2 antibody; Rattus norvegicus KLF2 antibody Q9ET58 92%
Rhesus macaque LOC719492 antibody; Macaca mulatta LOC719492 antibody F6YZ27 100%
Small-eared galago KLF2 antibody; Otolemur garnettii KLF2 antibody H0XV81 100%
Three-spined stickleback KLF2 (2 of 2) antibody; Gasterosteus aculeatus KLF2 (2 of 2) antibody G3PXH3 78%
Western clawed frog klf4 antibody; Xenopus tropicalis klf4 antibody A0JLZ3 90%
White-tufted-ear marmoset LOC100388178 antibody; Callithrix jacchus LOC100388178 antibody F7ACF8 92%
White-tufted-ear marmoset LOC100388178 antibody; Callithrix jacchus LOC100388178 antibody F7ACF4 92%
Zebra finch LOC100227115 antibody; Taeniopygia guttata LOC100227115 antibody H0YR03 78%
Zebrafish klf2a antibody; Danio rerio klf2a antibody Q6P3M5 78%
Zebrafish klf2a antibody; Danio rerio klf2a antibody Q1LXI7 78%
Zebrafish klf2b antibody; Danio rerio klf2b antibody Q90XE7 78%

Product Protocols: KLF2 antibody tested with Human 721_B_Lymphoblast (ARP32760_P050)

Aviva Systems Biology is the original manufacturer of this KLF2 antibody (ARP32760_P050)

Click here to view the KLF2 antibody Western Blot Protocol

Product Datasheet Link: KLF2 antibody (ARP32760_P050)

WB Suggested Anti-KLF2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B

Western Blot image:


Description of Target: KLF2 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 3 C2H2-type zinc fingers. KLF2 binds to the CACCC box in the beta-globin gene promoter and activates transcription.

Questions pertaining to this data can be directed to techsupport@avivasysbio.com

Aviva Systems Biology’s KLF2 antibody (ARP32760_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.

To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.

All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com

Product Review: KLF2 antibody - middle region (ARP32760_P050) in macrophage cell line J774A.1 using Western Blot

Product Page Link:  KLF2 antibody - middle region (ARP32760_P050)

Data Provided by Robyn Pilcher-Roberts, University of Cincinnati, Cincinnati, OH

How do Aviva's reagents play a role in your experimental goals?

Level of protein expression check

How many different experimental trials were conducted using the antibody sample? 3

What type of experimental sample are you using and how did you prepare it?

Mouse lung, liver and spleen

What were your antibody dilutions?

Primary: KLF2 antibody at 1:000

Secondary: Fluorescent Goat Anti-Rabbit at 1:5000

What controls were used in your experiment? Postive control was NIH373 cell lysate

How did you store the antibody after re-suspension? Aliquotted @ -20C

Protocol:

12% SDS-PAGE gel, approximately 12cm, ran @ 150V. Transfer @ 4C 100V for 3hrs. Block for 2hrs in TBS/.05% Tween/5% milk or 5% BSA, primary ON @ 4C and quick block again for 10minutes. Secondary for 1hr. @ room temperature. Visualize with LiCor Imager.

Do you plan tto use this antibody in future experiments or to publish your data? Yes, in process of now resubmitting a mansuscript which will include a western

Product Reviews: KLF2 antibody - middle region (ARP32760_P050) in HCT116 using Chromatin Immunoprecipitation

The product page for Aviva’s KLF2 antibody - middle region (ARP32760_P050) can be found here.

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

 

Protocol

Matrix-ChIP utilizes sheared chromatin. Capture antibodies are surface-immobilized via blocked, Protein A coated 96-well plates. Blocking buffer consists of 5% BSA and 100ug/mL sheared salmon sperm DNA in immunoprecipitation (IP) buffer. After washing, sheared chromatin samples are added and the plates are floated in an ultrasonic water bath (Bronson 8510) to accelerate protein-antibody binding. Wells are then washed and DNA eluted with Tris base (pH 9.8) and stored (4C) in the same Matrix ChIP plates for repeated use. Plates are sealed with adhesive film to prevent evaporation and can be stored/re-sealed for months for repeated qPCR.

Real-time PCR is performed with 2X SYBR Green PCR master mix, eluted DNA template and primers in a 2-4uL final volume in a 384-well Optical Reaction Plate. Amplification (three steps, 40 cycles), data acquisition and analysis were run in qudriplicate for each PCR reaction.

-Amount of chromatin input equivalent to 200-500ng DNA (100uL at 2-5ng/uL)
-Amount of antibody used: 0.5ug
-Fold molar excess of blocking peptide: 100
-Antibody:Blocking peptide incubation time: 30min at RT
-Antibody:chromatin incubation time: 60min at 4C in ultrasonic water bath (Branson 8510)
-ChIP assays done in duplicate
-All time points are in minutes

Microplate-based chromatin immunoprecipitation method, Matrix-ChIP: a platform to study signaling of complex genomic events.
Flanagin S, Nelson JD, Castner DG, Denisenko O, Bomsztyk K.
Nucleic Acids Res. 2008 Feb;36(3)

Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays.
Yu J, Feng Q, Ruan Y, Komers R, Kiviat N, Bomsztyk K.
BMC Mol Biol. 2011 Nov 18;12:49.

 
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