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Catalog No: OKEH02810
Size:96T
Price: $525.00
SKU
OKEH02810
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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KLF10 ELISA Kit (Human) : 96 Wells (OKEH02810)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KLF10 ELISA Kit (Human) (OKEH02810) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLF10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KLF10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLF10 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-KLF10 Microplate96 Wells (12 x 8 Well strips)
KLF10 Lyophilized Standard2
100X Biotinylated KLF10 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Krueppel-like factor 10
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKLF10
Gene Full NameKruppel like factor 10
Alias Symbolsearly growth response-alpha;EGRA;EGR-alpha;Krueppel-like factor 10;TGFB-inducible early growth response protein 1;TIEG;TIEG1;transforming growth factor-beta-inducible early growth response protein 1;zinc finger transcription factor TIEG.
NCBI Gene Id7071
Protein NameQ13118
Description of TargetKrueppel-like factor 10
Uniprot IDhttps://www.uniprot.org/uniprot/Q13118
Protein Accession #NP_001027453.1
Nucleotide Accession #NM_001032282.3
Protein Size (# AA)480
Molecular Weight53 kDa
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