Kitlg ELISA Kit (Rat) (OKEH04516)

Catalog No: OKEH04516

Size:96T

Rat Kitlg ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Kitlg ELISA Kit (Rat) (OKEH04516) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kitlg has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Kitlg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kitlg captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Kitlg Microplate: 96 Wells (12 x 8 Well Strips)
  • Kitlg Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Kitlg Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
• Sensitivity: 15.78 pg/mL
• Specificity: Natural and recombinant Rat Kit ligand
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Kitlg
• Gene Full Name: KIT ligand
• Alias Symbols: C-kit ligand;hematopoietic growth factor KL;kit ligand;Kitl;mast cell growth factor;Mgf;SCF;steel factor/kit ligand;Stem cell factor;stem cell factor KL-1.
• NCBI Gene Id: 60427
• Protein Name: P21581
• Description of Target: Kit ligand
• Uniprot ID: https://www.uniprot.org/uniprot/P21581
• Protein Accession #: NP_068615.1
• Nucleotide Accession #: NM_021843.4
• Protein Size (# AA): 273
• Molecular Weight: 31 KDa