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Catalog No: OKEH01462
Size:96T
Price: $525.00
SKU
OKEH01462
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KISS1 ELISA Kit (Mouse) (OKEH01462) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KISS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KISS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KISS1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.6% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
KISS1 Microplate96 Wells (12 x 8 Well strips)
KISS1 Lyophilized Standard2
100X Biotinylated KISS1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity40.4 pg/mL
SpecificityNatural and recombinant Mouse Metastasis-suppressor KiSS-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKiss1
Gene Full NameKiSS-1 metastasis-suppressor
Alias Symbols0610012C01Rik;kis;Kiss1 metastasis-suppressor;kisspeptin;kisspeptin-1;meta;metastasis-suppressor KiSS-1;metastatin;metastin.
NCBI Gene Id280287
Protein NameQ6Y4S4
Description of TargetMetastasis-suppressor KiSS-1
Uniprot IDhttps://www.uniprot.org/uniprot/Q6Y4S4
Protein Accession #NP_839991.2
Nucleotide Accession #NM_178260.3
Protein Size (# AA)130
Molecular Weight14 kDa
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