Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH04141
Size:96T
Price: $525.00
SKU
OKEH04141
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KIF5A ELISA Kit (Human) (OKEH04141) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KIF5A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KIF5A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KIF5A captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.6% (n = 20)
Mean Inter-assay CV%: <= 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-KIF5A Microplate96 Wells (12 x 8 Well Strips)
KIF5A Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
KIF5A Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Kinesin heavy chain isoform 5A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKIF5A
Gene Full Namekinesin family member 5A
Alias SymbolsALS25;D12S1889;KIF5A variant protein;kinesin heavy chain isoform 5A;kinesin heavy chain neuron-specific 1;kinesin, heavy chain, neuron-specific;MY050;NEIMY;neuronal kinesin heavy chain;NKHC;SPG10.
NCBI Gene Id3798
Protein NameQ12840
Description of TargetKinesin heavy chain isoform 5A
Uniprot IDhttps://www.uniprot.org/uniprot/Q12840
Protein Accession #NP_004975.2
Nucleotide Accession #NM_004984.2
Protein Size (# AA)1032
Molecular Weight117 kDa
Write Your Own Review
You're reviewing:KIF5A ELISA Kit (Human) (OKEH04141)
Your Rating
We found other products you might like!