KIF2C Antibody (OABB01675)

Catalog No: OABB01675

Size:100UG

Anti- MCAK antibody, OABB01675, Western blotting
All lanes: Anti MCAK (OABB01675) at 0.5ug/ml
Lane 1: Mouse Testis Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: MM453 Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 81 kDa
Observed bind size: 81 kDa

Anti- MCAK antibody, OABB01675, Western blotting
All lanes: Anti MCAK (OABB01675) at 0.5ug/ml
WB: Recombinant Human MCAK Protein 0.5ng
Predicted bind size: 48 kDa
Observed bind size: 48 kDa

Anti- MCAK antibody, OABB01675, IHC(P)
IHC(P): Mouse Testis Tissue

Anti- MCAK antibody, OABB01675, IHC(P)
IHC(P): Rat Testis Tissue

Anti- MCAK antibody, OABB01675, IHC(P)
IHC(P): Rat Thymus Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for MCAK is approximately 0.25ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse, Rat: By Heat
  Immunocytochemistry/Immunofluorescence: 2 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Kinesin-like protein KIF2C(KIF2C) detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: KIF2C
• Gene Full Name: kinesin family member 2C
• Alias Symbols: CT139;kinesin-like protein 6;kinesin-like protein KIF2C;KNSL6;MCAK;mitotic centromere-associated kinesin;testis tissue sperm-binding protein Li 68n.
• NCBI Gene Id: 11004
• Protein Name: Kinesin-like protein KIF2C
• Description of Target: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed:23891108).
• Uniprot ID: Q99661
• Molecular Weight: 81313 MW