Catalog No: OKEH01435
Size:96T
Price: $600.00
SKU
OKEH01435
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01435
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KHK ELISA Kit (Human) (OKEH01435) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KHK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KHK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KHK captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
KHK Microplate96 Wells (12 x 8 Well strips)
KHK Lyophilized Standard2
100X Biotinylated KHK Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Ketohexokinase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKHK
Gene Full Nameketohexokinase
Alias Symbolsfructokinase;hepatic fructokinase;ketohexokinase;testicular tissue protein Li 102.
NCBI Gene Id3795
Protein NameP50053
Description of TargetKetohexokinase
Uniprot IDhttps://www.uniprot.org/uniprot/P50053
Protein Accession #NP_000212.1
Nucleotide Accession #NM_000221.2
Protein Size (# AA)298
Molecular Weight33 kDa
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