Catalog No: OKEH00246
Size:96T
Price: $525.00
SKU
OKEH00246
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

KDR ELISA Kit (Rat) : 96 Wells (OKEH00246)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KDR ELISA Kit (Rat) (OKEH00246) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KDR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for KDR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KDR captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
KDR Microplate96 Wells (12 x 8 Well strips)
KDR Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated KDR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.1 pg/mL
SpecificityNatural and recombinant Rat Vascular endothelial growth factor receptor 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKdr
Gene Full Namekinase insert domain receptor
Alias Symbolsfetal liver kinase 1;FLK-1;FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2);FLK1 kinase insert domain receptor (VEGF receptor 2);kinase insert domain protein receptor;protein-tyrosine kinase receptor flk-1;vascular endothelial growth factor receptor 2;Vegfr-2.
NCBI Gene Id25589
Protein NameO08775
Description of TargetVascular endothelial growth factor receptor 2
Uniprot IDhttps://www.uniprot.org/uniprot/O08775
Protein Accession #NP_037194.1
Nucleotide Accession #NM_013062.1
Protein Size (# AA)1343
Molecular Weight150 kDa
Write Your Own Review
You're reviewing:KDR ELISA Kit (Rat) : 96 Wells (OKEH00246)
Your Rating
We found other products you might like!