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Datasheets/Manuals | Printable datasheet for KDR Antibody (OAAF01094) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human VEGFR2. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | VEGFR2 antibody detects endogenous levels of total VEGFR2 protein |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:40000 |
Reference | Terman B.I., Oncogene 6:1677-1683(1991). Mitola S., J. Virol. 74:344-353(2000). Holmqvist K., J. Biol. Chem. 279:22267-22275(2004) |
Gene Symbol | FLT4|KDR |
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Gene Full Name | fms related receptor tyrosine kinase 4|kinase insert domain receptor |
Alias Symbols | CD309;CHTD7;Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4;Fetal liver kinase 1;fetal liver kinase-1;FLK1;FLT-4;FLT41;fms related tyrosine kinase 4;fms-like tyrosine kinase 4;Kinase insert domain receptor;kinase insert domain receptor (a type III receptor tyrosine kinase);LMPH1A;LMPHM1;PCL;primary congenital lymphedema;protein-tyrosine kinase receptor Flk-1;soluble VEGFR2;tyrosine kinase growth factor receptor;tyrosine-protein kinase receptor FLT4;vascular endothelial growth factor receptor 2;vascular endothelial growth factor receptor 3;VEGF receptor-3;VEGFR;VEGFR2;VEGFR3;VEGFR-3. |
NCBI Gene Id | 2324|3791 |
Protein Name | Vascular endothelial growth factor receptor 2|Vascular endothelial growth factor receptor 3 |
Description of Target | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.|Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'. |
Uniprot ID | P35916|P35968 |
Protein Accession # | NP_002244.1 |
Molecular Weight | 151 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "KDR Antibody (OAAF01094)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "KDR Antibody (OAAF01094)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "KDR Antibody (OAAF01094)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "KDR Antibody (OAAF01094)"?
This target may also be called "CD309;CHTD7;Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4;Fetal liver kinase 1;fetal liver kinase-1;FLK1;FLT-4;FLT41;fms related tyrosine kinase 4;fms-like tyrosine kinase 4;Kinase insert domain receptor;kinase insert domain receptor (a type III receptor tyrosine kinase);LMPH1A;LMPHM1;PCL;primary congenital lymphedema;protein-tyrosine kinase receptor Flk-1;soluble VEGFR2;tyrosine kinase growth factor receptor;tyrosine-protein kinase receptor FLT4;vascular endothelial growth factor receptor 2;vascular endothelial growth factor receptor 3;VEGF receptor-3;VEGFR;VEGFR2;VEGFR3;VEGFR-3." in publications.
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What is the shipping cost for "KDR Antibody (OAAF01094)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "KDR Antibody (OAAF01094)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "KDR Antibody (OAAF01094)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "151 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "KDR Antibody (OAAF01094)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "FLT4|KDR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "FLT4|KDR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "FLT4|KDR"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "FLT4|KDR"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "FLT4|KDR"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "FLT4|KDR"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.