Catalog No: OKCD01071 (Formerly GWB-KBBPA6)
Size:96WELLS
Price: $750.00
SKU
OKCD01071
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Datasheets/ManualsPrintable datasheet for KAZALD1 ELISA Kit (Human) (OKCD01071)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-KAZALD1 Microplate96 Wells (12 x 8 Well Strips)
KAZALD1 Lyophilized Standard2 x 20 ng
100X Biotinylated KAZALD1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). No significant cross-reactivity or interference between Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolKAZALD1
Gene Full NameKazal type serine peptidase inhibitor domain 1
Alias Symbolsbone- and odontoblast-expressed gene 1;BONO1;FKSG28;FKSG40;IGFBP-related protein 10;IGFBP-rP10;kazal-type serine protease inhibitor domain-containing protein 1;novel kazal-type serine protease inhibitor domain and immunoglobulin domain containing protein.
NCBI Gene Id81621
Protein NameKazal-type serine protease inhibitor domain-containing protein 1
Description of TargetInvolved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly (By similarity).
Uniprot IDQ96I82
Protein Accession #NP_001306232
Nucleotide Accession #NM_001319303.1
Protein Size (# AA)304
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