Catalog No: OKCD01071 (Formerly GWB-KBBPA6)
Size:96WELLS
Price: $750.00
SKU
OKCD01071
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Email: info@avivasysbio.com
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Datasheets/Manuals | Printable datasheet for KAZALD1 ELISA Kit (Human) (OKCD01071) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.115 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). No significant cross-reactivity or interference between Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | KAZALD1 |
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Gene Full Name | Kazal type serine peptidase inhibitor domain 1 |
Alias Symbols | bone- and odontoblast-expressed gene 1;BONO1;FKSG28;FKSG40;IGFBP-related protein 10;IGFBP-rP10;kazal-type serine protease inhibitor domain-containing protein 1;novel kazal-type serine protease inhibitor domain and immunoglobulin domain containing protein. |
NCBI Gene Id | 81621 |
Protein Name | Kazal-type serine protease inhibitor domain-containing protein 1 |
Description of Target | Involved in the proliferation of osteoblasts during bone formation and bone regeneration. Promotes matrix assembly (By similarity). |
Uniprot ID | Q96I82 |
Protein Accession # | NP_001306232 |
Nucleotide Accession # | NM_001319303.1 |
Protein Size (# AA) | 304 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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