Catalog No: OKEH04812
Size:96T
Price: $525.00
SKU
OKEH04812
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Jun ELISA Kit (Rat) (OKEH04812) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Jun has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Jun is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Jun captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 7.8-500ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
Sensitivity | 4.05 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat Transcription factor AP-1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Jun |
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Gene Full Name | Jun proto-oncogene, AP-1 transcription factor subunit |
Alias Symbols | activator protein 1;AP1;Avian sarcoma virus 17 (v-jun) oncogene homolog;Jun oncogene;proto-oncogene c-jun;transcription factor AP-1;V-jun avian sarcoma virus 17 oncogene homolog;v-jun sarcoma virus 17 oncogene homolog. |
NCBI Gene Id | 24516 |
Protein Name | P17325 |
Description of Target | Transcription factor AP-1 |
Uniprot ID | https://www.uniprot.org/uniprot/P17325 |
Protein Accession # | NP_068607.1 |
Nucleotide Accession # | NM_021835.3 |
Protein Size (# AA) | 334 |
Molecular Weight | 36 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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