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Catalog No: OKEH04812
Size:96T
Price: $525.00
SKU
OKEH04812
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Jun ELISA Kit (Rat) (OKEH04812) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Jun has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Jun is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Jun captured in well.
ELISA Kit Range7.8-500ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Jun Microplate96 Wells (12 x 8 Well Strips)
Jun Standard2
Sample Diluent1 x 20 mL
Jun Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity4.05 ng/mL
SpecificityNatural and recombinant Rat Transcription factor AP-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolJun
Gene Full NameJun proto-oncogene, AP-1 transcription factor subunit
Alias Symbolsactivator protein 1;AP1;Avian sarcoma virus 17 (v-jun) oncogene homolog;Jun oncogene;proto-oncogene c-jun;transcription factor AP-1;V-jun avian sarcoma virus 17 oncogene homolog;v-jun sarcoma virus 17 oncogene homolog.
NCBI Gene Id24516
Protein NameP17325
Description of TargetTranscription factor AP-1
Uniprot IDhttps://www.uniprot.org/uniprot/P17325
Protein Accession #NP_068607.1
Nucleotide Accession #NM_021835.3
Protein Size (# AA)334
Molecular Weight36 kDa
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