JAK3 Recombinant Protein (OPCD04634)

Catalog No: OPCD04634

JAK3 Recombinant Protein (OPCD04634) using SDS-PAGE

JAK3 Recombinant Protein (OPCD04634) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Trp716-Asp967
  Subcellular Location: Membrane, Cytoplasm, Endoplasmic reticulum lumen
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 97%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: JAK3
• Gene Full Name: Janus kinase 3
• Alias Symbols: JAK-3;JAK3_HUMAN;JAKL;Janus kinase 3;Janus kinase 3 (a protein tyrosine kinase, leukocyte);leukocyte Janus kinase;LJAK;L-JAK;tyrosine-protein kinase JAK3.
• NCBI Gene Id: 3718
• Protein Name: Tyrosine-protein kinase JAK3
• Description of Target: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
• Uniprot ID: P52333
• Protein Accession #: NP_000206.2
• Nucleotide Accession #: NM_000215.3
• Protein Size (# AA): Trp716~Asp967
• Molecular Weight: 32kDa