JAK3 ELISA Kit (Rat) (OKEH04318)

Catalog No: OKEH04318

Size:96T

Rat JAK3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Jak3 ELISA Kit (Rat) (OKEH04318) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Jak3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Jak3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Jak3 captured in the well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Component:
  • Jak3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Jak3 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Jak3 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.426 ng/mL
• Specificity: Natural and recombinant Rat Tyrosine-protein kinase JAK3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Jak3
• Gene Full Name: Janus kinase 3
• Alias Symbols: JAK-3;Janus kinase 3;Janus kinase 3, protein-tyrosine kinase;RATJAK3;tyrosine-protein kinase JAK3.
• NCBI Gene Id: 25326
• Protein Name: Q63272
• Description of Target: Tyrosine-protein kinase JAK3
• Uniprot ID: https://www.uniprot.org/uniprot/Q63272
• Protein Accession #: NP_036987.2
• Nucleotide Accession #: NM_012855.2
• Protein Size (# AA): 1100
• Molecular Weight: 123 kDa