JAK2 Antibody - C-terminal region (OAAB07505)

Catalog No: OAAB07505

Size:400ul

The anti-JAK2 Pab (Cat. #OAAB07505) is used in Western blot to detect JAK2 in mouse thymus tissue lysate.

Confocal immunofluorescent analysis of JAK2 Antibody (C-term) (Cat#OAAB07505) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

JAK2 Antibody (C-term) (Cat. #OAAB07505) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF, FC
• Additional Information: Precautions: JAK2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin
  Cellular Location: Endomembrane system; Peripheral membrane protein (By similarity). Cytoplasm. Nucleus
  Tissue Location: Ubiquitously expressed throughout most tissues
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This JAK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1101-1132 amino acids from the C-terminal region of human JAK2.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: JAK2
• Alias Symbols: JTK10
• NCBI Gene Id: 3717
• Protein Name: Tyrosine-protein kinase JAK2
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O60674
• Protein Accession #: NP_004963.1
• Molecular Weight: 130674 Da