JAG1 ELISA Kit (Human) (OKCD02666)

Catalog No: OKCD02666

Size:96WELLS

Human JAG1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	81-95%	87-98%	80-92%
  EDTA Plasma (n=5)	97-104%	84-98%	85-98%	91-105%
  Heparin Plasma (n=5)	92-103%	91-102%	96-104%	78-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jagged 1 (JAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Jagged 1 (JAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Jagged 1 (JAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Jagged 1 (JAG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-103	93
  EDTA Plasma (n=5)	96-104	99
  Heparin Plasma(n=5)	87-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Jagged 1 (JAG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Jagged 1 (JAG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-JAG1 Microplate: 96 Wells (12 x 8 Well strips)
  • JAG1 Lyophilized Standard: 2
  • 100X Biotinylated JAG1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Jagged 1 Protein (JAG1). No significant cross-reactivity or interference between Jagged 1 Protein (JAG1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: JAG1
• Gene Full Name: jagged canonical Notch ligand 1
• Alias Symbols: AGS;AGS1;AHD;AWS;CD339;DCHE;HJ1;JAGL1;protein jagged-1.
• NCBI Gene Id: 182
• Protein Name: Protein jagged-1
• Description of Target: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
• Uniprot ID: P78504
• Protein Accession #: NP_000205.1
• Nucleotide Accession #: NM_000214.2
• Protein Size (# AA): 1218