ITPA ELISA Kit (Mouse) (OKCD02665)

Catalog No: OKCD02665

Size:96WELLS

Mouse ITPA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inosine Triphosphatase Pyrophosphatase (ITPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inosine Triphosphatase Pyrophosphatase (ITPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inosine Triphosphatase Pyrophosphatase (ITPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inosine Triphosphatase Pyrophosphatase (ITPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inosine Triphosphatase Pyrophosphatase (ITPA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inosine Triphosphatase Pyrophosphatase (ITPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ITPA Microplate: 96 Wells (12 x 8 Well strips)
  • ITPA Lyophilized Standard: 2
  • 100X Biotinylated ITPA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell Lysates and other biological fluids
• Sensitivity: 0.049 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Inosine Triphosphatase (ITPA). No significant cross-reactivity or interference between Inosine Triphosphatase (ITPA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Itpa
• Gene Full Name: inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
• Alias Symbols: 2010016I08Rik;AU020102;I;inosine triphosphate pyrophosphatase;Itp;ITPase;non-canonical purine NTP pyrophosphatase;non-standard purine NTP pyrophosphatase;NTPase;nucleoside triphosphate pyrophosphatase;nucleoside-triphosphate diphosphatase;Nucleoside-triphosphate pyrophosphatase.
• NCBI Gene Id: 16434
• Protein Name: Inosine triphosphate pyrophosphatase
• Description of Target: Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
• Uniprot ID: Q9D892
• Protein Accession #: NP_080198.2
• Nucleotide Accession #: NM_025922.2
• Protein Size (# AA): 198