ITLN1 ELISA Kit (Mouse) (OKCD02664)

Catalog No: OKCD02664

Size:96WELLS

Mouse ITLN1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-105%	98-105%	98-105%	85-92%
  EDTA Plasma (n=5)	84-91%	93-101%	83-98%	80-101%
  Heparin Plasma (n=5)	87-94%	80-94%	99-105%	83-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intelectin 1 (ITLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intelectin 1 (ITLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intelectin 1 (ITLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Intelectin 1 (ITLN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.5-800pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-91	82
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma(n=5)	85-98	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intelectin 1 (ITLN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intelectin 1 (ITLN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ITLN1 Microplate: 96 Wells (12 x 8 Well strips)
  • ITLN1 Lyophilized Standard: 2
  • 100X Biotinylated ITLN1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Intelectin 1 (ITLN1). No significant cross-reactivity or interference between Intelectin 1 (ITLN1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Itln1
• Gene Full Name: intelectin 1 (galactofuranose binding)
• Alias Symbols: calcium-dependent galactosyl-specific lectin;galactofuranose-binding lectin;I;intelectin 2;intelectin 5;intelectin a;intelectin-1a;intestinal lactoferrin receptor;IntL;It;Itln;Itln2;Itln3;Itln5;Itlna;Lfr;mL;truncated intelectin 3.
• NCBI Gene Id: 16429
• Protein Name: Intelectin-1a
• Description of Target: Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed:26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris. Does not bind mammalian glycans. Probably plays a role in the defense system against microorganisms. May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (By similarity).
• Uniprot ID: O88310
• Protein Accession #: NP_034714.1
• Nucleotide Accession #: NM_010584.3
• Protein Size (# AA): 313