ITK Antibody (OAEB01818)

Catalog No: OAEB01818 (Formerly GWB-EB094A)

Size:100UG

OAEB01818 (1ug/ml) staining of Jurkat nuclear cell lysate (A) and negative control Human parathyroid gland (B) (35 ugg protein in RIPA buffer). Detected by chemiluminescence.

OAEB01818 - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

OAEB01818 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong cytoplasmic and weak nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IF
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: ITK
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Peptide Sequence: C-RLLRQLAEIAESGL
• Concentration: 0.5 mg/ml
• Application Info: Peptide ELISA: antibody detection limit dilution 1:2000.
  Western blot: Approx 75kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8kDa according to NP_005537.3). Recommended concentration: 1-3g/ml. Primary incubation 1 hour at room temperature.
  Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10µg/ml.
  Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentration:of 10ug/ml.
• Reference: * Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K.; A novel human tyrosine kinase gene inducible in T cells by interleukin 2.; FEBS Lett 1993 Jun 7;324(1):1-5; Pubmed 8504851

Target Info

• Gene Symbol: Itk|ITK
• Gene Full Name: IL2 inducible T cell kinase
• Alias Symbols: Emt;homolog of mouse T-cell itk/tsk;IL-2-inducible T-cell kinase;interleukin-2-inducible T cell kinase;interleukin-2-inducible T-cell kinase;kinase EMT;kinase TLK;LPFS1;LYK;PSCTK2;T-cell-specific kinase;Tcs;Tcsk;Ts;Tsk;tyrosine-protein kinase ITK/TSK;tyrosine-protein kinase LYK.
• NCBI Gene Id: 16428|3702
• Protein Name: Tyrosine-protein kinase ITK/TSK
• Uniprot ID: Q08881
• Protein Accession #: NP_005537.3
• Molecular Weight: 71.8