Catalog No: OKEH03926
Size:96T
Price: $625.00
SKU
OKEH03926
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ITIH4 ELISA Kit (Pig) (OKEH03926) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITIH4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITIH4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITIH4 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
ITIH4 Microplate96 Wells (12 x 8 Well strips)
ITIH4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ITIH4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.092 ng/mL
SpecificityNatural and recombinant Pig Inter-alpha-trypsin inhibitor heavy chain H4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolITIH4
Gene Full Nameinter-alpha-trypsin inhibitor heavy chain 4
Alias SymbolsIHRP;inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein);inter-alpha-inhibitor heavy chain 4;inter-alpha-trypsin inhibitor family heavy chain-related protein;inter-alpha-trypsin inhibitor heavy chain family member 4;inter-alpha-trypsin inhibitor heavy chain H4;ITI heavy chain H4;ITI-HC4;major acute phase protein;MAP.
NCBI Gene Id396799
Protein NameP79263
Description of TargetInter-alpha-trypsin inhibitor heavy chain H4
Uniprot IDhttps://www.uniprot.org/uniprot/P79263
Protein Accession #NP_001001537.1
Nucleotide Accession #NM_001001537.1
Protein Size (# AA)921
Molecular Weight921 kDa
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