Catalog No: OKCD02663
Size:96WELLS
Price: $700.00
SKU
OKCD02663
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Datasheets/ManualsPrintable datasheet for ITIH4 ELISA Kit (Mouse) (OKCD02663)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-98%86-98%85-103%81-103%
EDTA Plasma (n=5)81-99%97-104%85-93%91-98%
Heparin Plasma (n=5)78-94%80-89%79-101%93-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H4 (ITIH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inter Alpha-Globulin Inhibitor H4 (ITIH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10395
EDTA Plasma (n=5)89-10295
Heparin Plasma(n=5)82-9788
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H4 (ITIH4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H4 (ITIH4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ITIH4 Microplate96 Wells (12 x 8 Well strips)
ITIH4 Lyophilized Standard2
100X Biotinylated ITIH4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity3.7 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Inter Alpha-Globulin Inhibitor H4 (ITIH4). No significant cross-reactivity or interference between Inter Alpha-Globulin Inhibitor H4 (ITIH4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolItih4
Gene Full Nameinter alpha-trypsin inhibitor, heavy chain 4
Alias Symbolsinter alpha-trypsin inhibitor, heavy chain 4;inter-alpha-inhibitor heavy chain 4;ITI heavy chain H4;Itih;Itih-4;ITI-HC4;PK-120.
NCBI Gene Id16427
Protein NameInter alpha-trypsin inhibitor, heavy chain 4
Description of TargetType II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
Uniprot IDA6X935
Protein Accession #NP_001152771.1
Nucleotide Accession #NM_001159299.2
Protein Size (# AA)942
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