Catalog No: OKEH03639
Size:96T
Price: $625.00
SKU
OKEH03639
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ITIH3 ELISA Kit (Human) (OKEH03639) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ITIH3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ITIH3 and incubated. The ITIH3 found in the sample or standards competes with the biotinylated ITIH3 for limited binding sites on the immobilized anti-human ITIH3 antibody. Excess unbound biotinylated ITIH3 and sample or standard ITIH3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ITIH3 captured in the well and inversely proportional to the amount of ITIH3 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88.9%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.9% (n = 20)
Mean Inter-assay CV%: <= 11.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-ITIH3 Microplate96 Wells (12 x 8 Well strips)
ITIH3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X ITIH3-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.19 ng/mL
SpecificityNatural and recombinant Human Inter-alpha-trypsin inhibitor heavy chain H3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolITIH3
Gene Full Nameinter-alpha-trypsin inhibitor heavy chain 3
Alias SymbolsH3P;inter-alpha (globulin) inhibitor H3;inter-alpha (globulin) inhibitor, H3 polypeptide;inter-alpha-inhibitor heavy chain 3;inter-alpha-trypsin inhibitor heavy chain H3;ITI heavy chain H3;ITI-HC3;pre-alpha (globulin) inhibitor, H3 polypeptide;serum-derived hyaluronan-associated protein;SHAP.
NCBI Gene Id3699
Protein NameQ06033
Description of TargetInter-alpha-trypsin inhibitor heavy chain H3
Uniprot IDhttps://www.uniprot.org/uniprot/Q06033
Protein Accession #NP_002208.3
Nucleotide Accession #NM_002217.3
Protein Size (# AA)890
Molecular Weight100 kDa
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