Catalog No: OKEH03639
Size:96T
Price: $625.00
SKU
OKEH03639
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology ITIH3 ELISA Kit (Human) (OKEH03639) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ITIH3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ITIH3 and incubated. The ITIH3 found in the sample or standards competes with the biotinylated ITIH3 for limited binding sites on the immobilized anti-human ITIH3 antibody. Excess unbound biotinylated ITIH3 and sample or standard ITIH3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ITIH3 captured in the well and inversely proportional to the amount of ITIH3 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88.9% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 6.9% (n = 20) Mean Inter-assay CV%: <= 11.7% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.19 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Inter-alpha-trypsin inhibitor heavy chain H3 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | ITIH3 |
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Gene Full Name | inter-alpha-trypsin inhibitor heavy chain 3 |
Alias Symbols | H3P;inter-alpha (globulin) inhibitor H3;inter-alpha (globulin) inhibitor, H3 polypeptide;inter-alpha-inhibitor heavy chain 3;inter-alpha-trypsin inhibitor heavy chain H3;ITI heavy chain H3;ITI-HC3;pre-alpha (globulin) inhibitor, H3 polypeptide;serum-derived hyaluronan-associated protein;SHAP. |
NCBI Gene Id | 3699 |
Protein Name | Q06033 |
Description of Target | Inter-alpha-trypsin inhibitor heavy chain H3 |
Uniprot ID | https://www.uniprot.org/uniprot/Q06033 |
Protein Accession # | NP_002208.3 |
Nucleotide Accession # | NM_002217.3 |
Protein Size (# AA) | 890 |
Molecular Weight | 100 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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