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Catalog No: OKEH01079
Size:96T
Price: $525.00
SKU
OKEH01079
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ITGA4 ELISA Kit (Mouse) : 96 Wells (OKEH01079)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ITGA4 ELISA Kit (Mouse) (OKEH01079) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITGA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITGA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITGA4 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.5% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
ITGA4 Microplate96 Wells (12 x 8 Well strips)
ITGA4 Lyophilized Standard2
100X Biotinylated ITGA4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Mouse Integrin alpha-4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolItga4
Gene Full Nameintegrin alpha 4
Alias SymbolsCD49 antigen-like family member D;CD49D;integrin alpha-4;integrin alpha4B;integrin alpha-IV;Itga4B;LPAM subunit alpha;lymphocyte Peyer patch adhesion molecules subunit alpha;VLA-4 receptor, alpha 4 subunit;VLA-4 subunit alpha.
NCBI Gene Id16401
Protein NameQ00651
Description of TargetIntegrin alpha-4
Uniprot IDhttps://www.uniprot.org/uniprot/Q00651
Protein Accession #NP_034706.3
Nucleotide Accession #NM_010576.3
Protein Size (# AA)1039
Molecular Weight116 kDa
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