ITGA2 ELISA Kit (Human) (OKCD00270)

Catalog No: OKCD00270 (Formerly GWB-KBBXW6)

Size:96WELLS

Human ITGA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 2 (ITGa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 2 (ITGa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 2 (ITGa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Integrin Alpha 2 (ITGa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha 2 (ITGa2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha 2 (ITGa2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ITGA2 Microplate: 96 Wells (12 x 8 Well Strips)
  • ITGA2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated ITGA2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 2 (ITGa2). No significant cross-reactivity or interference between Integrin Alpha 2 (ITGa2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ITGA2
• Gene Full Name: integrin subunit alpha 2
• Alias Symbols: alpha 2 subunit of VLA-2 receptor;BR;CD49 antigen-like family member B;CD49B;collagen receptor;GPIa;HPA-5;human platelet alloantigen system 5;integrin alpha-2;integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor);platelet antigen Br;platelet glycoprotein GPIa;platelet membrane glycoprotein Ia;very late activation protein 2 receptor, alpha-2 subunit;VLA-2;VLA-2 subunit alpha;VLAA2.
• NCBI Gene Id: 3673
• Protein Name: Integrin alpha-2
• Description of Target: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
• Uniprot ID: P17301
• Protein Accession #: NP_002194
• Nucleotide Accession #: NM_002203.3
• Protein Size (# AA): 1181