ITGA1 ELISA Kit (Mouse) (OKCD01198)

Catalog No: OKCD01198 (Formerly GWB-KBBCE9)

Size:96WELLS

Mouse Itga1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 1 (ITGa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 1 (ITGa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 1 (ITGa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Integrin Alpha 1 (ITGa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78.12-5000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha 1 (ITGa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Itga1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Itga1 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated Itga1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 31 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 1 (ITGa1). No significant cross-reactivity or interference between Integrin Alpha 1 (ITGa1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Itga1
• Gene Full Name: integrin alpha 1
• Alias Symbols: CD49;CD49 antigen-like family member A;CD49A;E130012M19Rik;integrin alpha-1;laminin and collagen receptor;V;Vla1;VLA-1.
• NCBI Gene Id: 109700
• Protein Name: Integrin alpha-1
• Description of Target: Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.
• Uniprot ID: Q3V3R4
• Protein Accession #: NP_001028400
• Nucleotide Accession #: NM_001033228.3
• Protein Size (# AA): 1179