- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OAAB07829 |
---|
Predicted Species Reactivity | Human, Mouse |
---|---|
Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, FC |
Additional Information | Precautions: ISWI Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
:: | Function: Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing |
:: | Cellular Location: Nucleus. Tissue Location: Ubiquitously expressed. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This ISWI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 946-975 amino acids from the C-terminal region of human ISWI. |
Predicted Homology Based on Immunogen Sequence | Human; Mouse |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:1000 FC: 1:10~50 |
Gene Symbol | SMARCA5 |
---|---|
Alias Symbols | ISWI, SNF2H, hISWI, hSNF2H, WCRF135 |
NCBI Gene Id | 8467 |
Protein Name | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 |
Description of Target | Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Uniprot ID | O60264 |
Protein Accession # | NP_003592.3 |
Molecular Weight | 121905 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "ISWI Antibody - C-terminal region (OAAB07829)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
-
How long will it take to receive "ISWI Antibody - C-terminal region (OAAB07829)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
-
What buffer format is "ISWI Antibody - C-terminal region (OAAB07829)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ISWI Antibody - C-terminal region (OAAB07829)"?
This target may also be called "ISWI, SNF2H, hISWI, hSNF2H, WCRF135" in publications.
-
What is the shipping cost for "ISWI Antibody - C-terminal region (OAAB07829)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "ISWI Antibody - C-terminal region (OAAB07829)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "ISWI Antibody - C-terminal region (OAAB07829)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "121905 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ISWI Antibody - C-terminal region (OAAB07829)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "SMARCA5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "SMARCA5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "SMARCA5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "SMARCA5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "SMARCA5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "SMARCA5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.