Catalog No: OKDD00345 (Formerly GWB-KBBCP6)
Size:96 Wells
Price: $700.00
SKU
OKDD00345
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Datasheets/ManualsPrintable datasheet for IRF9 ELISA Kit (Human) (OKDD00345)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology IRF9 ELISA Kit (Human) (OKDD00345) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for IRF9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IRF9 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IRF9 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IRF9 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IRF9 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-IRF9 Microplate96 Wells (12 x 8 Well Strips)
IRF9 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X IRF9 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity< 0.042 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of IRF9. No significant cross-reactivity or interference between IRF9 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolIRF9
Gene Full Nameinterferon regulatory factor 9
Alias SymbolsIFN-alpha-responsive transcription factor subunit;interferon regulatory factor 9;interferon-stimulated gene factor 3 gamma;interferon-stimulated transcription factor 3, gamma (48kD);interferon-stimulated transcription factor 3, gamma 48kDa;IRF-9;ISGF3;ISGF-3 gamma;ISGF3 p48 subunit;ISGF3G;p48;transcriptional regulator ISGF3 subunit gamma.
NCBI Gene Id10379
Protein NameInterferon regulatory factor 9
Description of TargetTranscription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Uniprot IDQ00978
Protein Accession #NP_006075.3
Nucleotide Accession #NM_006084.4
Protein Size (# AA)393
Molecular Weight44 kDa
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