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Datasheets/Manuals | Printable datasheet for IRF5 Antibody (OAEB02490) |
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Predicted Species Reactivity | Bovine|Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
Additional Information | Reactivity Info: Tested: Human; Expected: Human, Mouse, Rat, Cow |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | IRF5 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 91%; Dog: 75%; Guinea Pig: 91%; Horse: 91%; Human: 100%; Mouse: 91%; Rabbit: 83%; Rat: 91% |
Peptide Sequence | C-QGPWPMHPAGMQ |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 57kDa band observed in lysates of cell line A549. In transfected HEK293 transiently expressing IRF5 a strong band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3ug/ml. |
Reference | * Hu G, Barnes BJ.; IRF-5 is a mediator of the death receptor-induced apoptotic signaling pathway.; J Biol Chem. 2009 Jan 30;284(5):2767-77.; Pubmed 19028697 * Ye L, Peng JS, Wang X, Wang YJ, Luo GX, Ho WZ.; Methamphetamine enhances Hepatitis C virus replication in human hepatocytes.; J Viral Hepat. 2008 Apr;15(4):261-70.; Pubmed 18307590 * Graham RR, Kyogoku C, Sigurdsson S, Vlasova IA, Davies LR, Baechler EC, Plenge RM, Koeuth T, Ortmann WA, Hom G, Bauer JW, Gillett C, Burtt N, Cunninghame Graham DS, Onofrio R, Petri M, Gunnarsson I, Svenungsson E, Ronnblom L, Nordmark G,; Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus.; Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6758-63.; Pubmed 17412832 * Ning S, Huye LE, Pagano JS.; Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit.; J Virol. 2005 Sep;79(18):11671-6.; Pubmed 16140744 * Barnes BJ, Moore PA, Pitha PM.; Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes.; J Biol Chem. 2001 Jun 29;276(26):23382-90.; Pubmed 11303025 |
Gene Symbol | IRF5 |
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Gene Full Name | interferon regulatory factor 5 |
Alias Symbols | interferon regulatory factor 5;SLEB10. |
NCBI Gene Id | 3663 |
Protein Name | Interferon regulatory factor 5 |
Uniprot ID | Q13568 |
Protein Accession # | NP_002191.1 |
Molecular Weight | 56.0; 56.0; 57.8 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "IRF5 Antibody (OAEB02490)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse|Rat".
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How long will it take to receive "IRF5 Antibody (OAEB02490)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "IRF5 Antibody (OAEB02490)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "IRF5 Antibody (OAEB02490)"?
This target may also be called "interferon regulatory factor 5;SLEB10." in publications.
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What is the shipping cost for "IRF5 Antibody (OAEB02490)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "IRF5 Antibody (OAEB02490)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "IRF5 Antibody (OAEB02490)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "56.0; 56.0; 57.8".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "IRF5 Antibody (OAEB02490)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "IRF5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "IRF5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "IRF5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "IRF5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "IRF5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "IRF5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.