IRF3 ELISA Kit (Mouse) (OKEH04478)

Catalog No: OKEH04478

Size:96T

Mouse IRF3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Irf3 ELISA Kit (Mouse) (OKEH04478) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Irf3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Irf3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Irf3 captured in the well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 8.3% (n = 20)
  Mean Inter-assay CV%: <= 10.3% (n = 20)
• ELISA Kit Component:
  • Anti-Irf3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Irf3 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Irf3 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 15.6 pg/mL
• Specificity: Natural and recombinant Mouse Interferon regulatory factor 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Irf3
• Gene Full Name: interferon regulatory factor 3
• Alias Symbols: C920001K05Rik;interferon regulatory factor 3;IRF;IRF-3.
• NCBI Gene Id: 54131
• Protein Name: P70671
• Description of Target: Interferon regulatory factor 3
• Uniprot ID: https://www.uniprot.org/uniprot/P70671
• Protein Accession #: NP_058545.1
• Nucleotide Accession #: NM_016849.4
• Protein Size (# AA): 419
• Molecular Weight: 47 KDa