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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for anti-IRF3 antibody - OAPA00270 |
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Predicted Species Reactivity | Human |
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Product Format | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
Clonality | Monoclonal |
Clone | P3F10AT. |
Isotype | Mouse IgG1 heavy chain and kappa light chain. |
Host | Mouse |
Application | WB |
:: | Product Type: Mouse Anti Human Monoclonal. |
:: | Product Introduction: Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is a member of the IRF family, and is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection. IRF-3 mediates interferon-stimulated response element (isre) promoter activation. Functions as a molecular switch for antiviral activity. Dsrna generated during the course of an viral infection leads to IRF3 phosphorylation on the c-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with creb binding protein (crebbp) to form dsrna-activated factor 1 (draf1), a complex which activates the transcription of genes under the control of isre. The complex binds to the ie and prdiii regions on the ifn |
Reconstitution and Storage | For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles. Stability: 12 months at -20C. 1 month at 4C. |
Immunogen | The immunogen for anti-IRF3 antibody: anti-human IRF3 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human IRF3 amino acids 108-166 purified from E. coli. |
Purity | IRF3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
Application Info | IRF3 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. |
Gene Symbol | IRF3 |
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Alias Symbols | IIAE7 |
NCBI Gene Id | 3661 |
Protein Name | Interferon regulatory factor 3 |
Description of Target | Mouse Anti Human Interferon Regulatory Factor-3 |
Uniprot ID | Q14653 |
Protein Accession # | NP_001184053.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "IRF3 Antibody (OAPA00270)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "IRF3 Antibody (OAPA00270)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "IRF3 Antibody (OAPA00270)" provided in?
This item is provided in "1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "IRF3 Antibody (OAPA00270)"?
This target may also be called "IIAE7" in publications.
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What is the shipping cost for "IRF3 Antibody (OAPA00270)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "IRF3 Antibody (OAPA00270)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "IRF3 Antibody (OAPA00270)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "IRF3 Antibody (OAPA00270)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "IRF3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "IRF3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "IRF3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "IRF3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "IRF3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "IRF3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.