IRF3 Antibody (OAAF00779)

Catalog No: OAAF00779 (Formerly GWB-ASC193)

Size:100 ug

Western blot analysis of extracts from K562 cells, using IRF3 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using IRF3 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using IRF3 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human IRF3.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: IRF-3 (Ab-385) antibody detects endogenous levels of total IRF-3 protein
• Application Info: WB 1:500~1:1000
  IHC 1:50~1:100
  IF 1:100~1:500
  ELISA 1:5000
• Reference: Wen-Shuz Yeow, J. Virol., Mar 2001; 75: 3021 - 3027.
  Subhra K. Biswas, Blood, Mar 2006; 107: 2112 - 2122.
  Benjamin R. tenOever, J. Virol., Apr 2002; 76: 3659 - 3669
• Publications:

  Dong, X.-Y. et al. Classical swine fever virus triggers RIG-I and MDA5-dependent signaling pathway to IRF-3 and NF-κB activation to promote secretion of interferon and inflammatory cytokines in porcine alveolar macrophages. Virol. J. 10, 286 (2013). 24034559

Target Info

• Gene Symbol: IRF3
• Gene Full Name: interferon regulatory factor 3
• Alias Symbols: IIAE7;interferon regulatory factor 3.
• NCBI Gene Id: 3661
• Protein Name: Interferon regulatory factor 3
• Description of Target: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
• Uniprot ID: Q14653
• Protein Accession #: NP_001184053.1
• Molecular Weight: 47 kDa