IRAK Antibody (OAPB01363)

Catalog No: OAPB01363

Size:100UG

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Monoclonal
• Host: IRAK monoclonal antibody was raised in mouse.
  Please use anti-mouse secondary antibodies.
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: IRAK monoclonal antibody can be stored at 4C, stable for one year.
• Immunogen: Recombinant protein corresponding to amino acids 618 - 712 of human IRAK.
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Application Info: IRAK antibody can be used for Western blot at 1 - 2 ug/mL. (Optimal dilution should be determined by user)
• Reference: Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.;Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.;Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.;Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.

Target Info

• Gene Symbol: IRAK1
• Gene Full Name: interleukin 1 receptor associated kinase 1
• Alias Symbols: interleukin-1 receptor-associated kinase 1;IRAK;IRAK-1;pelle;Pelle homolog.
• NCBI Gene Id: 3654
• Protein Name: Interleukin-1 receptor-associated kinase 1
• Description of Target: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
• Uniprot ID: P51617