Insulin ELISA Kit (Goat) : 96 Wells (OKCA00271)

Catalog No: OKCA00271

Size:96 Wells

Product Info

• Predicted Species Reactivity: Goat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	95%	90-101%
  Serum (n=4)	1:2	90%	80-94%
  Serum (n=4)	1:4	98%	90-105%
  Serum (n=4)	1:8	90%	80-96%

• ELISA Kit Principle: Aviva Systems Biology Insulin ELISA Kit (Goat) (OKCA00271) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Goat monoclonal antibody specific for Goat Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Goat Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Goat Insulin captured in well.
• ELISA Kit Range: 8 uIU/mL - 140 uIU/mL
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (μIU/mL)	33.77	32.486	30.447	30.913	31.656	32.302
  SD	0.02	0.022	0.019	0.027	0.026	0.029
  CV%	5.612	6.274	5.889	8.211	7.654	8.369

• ELISA Kit Component:
  • Anti-Insulin Microplate: 96 Well (12 x 8 Well Strips)
  • Insulin Standard: 5 x 500 uL
  • HRP-Conjugate: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: 5 uIU/ml
• Specificity: This assay has high sensitivity and excellent specificity for detection of goat INS. No significant cross-reactivity or interference between goat INS and analogues was observed
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Insulin
• Protein Name: Insulin
• Description of Target: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.
• Uniprot ID: P01319
• Protein Size (# AA): 51
• Molecular Weight: 6 kDa