Catalog No: OKCD01296
Size:96WELLS
Price: $800.00
SKU
OKCD01296
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Insulin Auto-Antibody ELISA Kit (Human) (OKCD01296) |
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Predicted Species Reactivity | Human | ||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||
ELISA Kit Duration | 2h, 30min | ||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Insulin Autoantibody (IAA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Autoantibody (IAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||
ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Autoantibody (IAA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Autoantibody (IAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||
Sensitivity | 1.15 ng/mL | ||||||||||||||||||||
Assay Info | Assay Method: Quantitative Reverse Capture Sandwich ELISA |
Alias Symbols | INSR, IDDM2, ILPR, Insulin, IRDN, MODY10 |
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Protein Name | Insulin Autoantibody |
Description of Target | Insulin Auto-antibody |
Uniprot ID | P01308 |
Nucleotide Accession # | NM_000207.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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