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Catalog No: OKEH03057
Size:96T
Price: $525.00
SKU
OKEH03057
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology INSL3 ELISA Kit (Rat) (OKEH03057) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Insl3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Insl3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Insl3 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay Precision - < 4.5%, n = 20
Mean Inter-Assay Precision - < 8.0%, n = 20
ELISA Kit Component
ComponentAmount
Anti-Insl3 Microplate96 Wells (12 x 8 Well strips)
Insl3 Lyophilized Standard2
100X Biotinylated Insl3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.082 ng/mL
SpecificityNatural and recombinant Rat Insulin-like 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolInsl3
Gene Full Nameinsulin-like 3
Alias Symbolsinsulin-like 3;Leydig insulin-like peptide;Leydig insulin-like peptide relaxin-like factor;ley-I-L;relaxin-like factor;Rlf.
NCBI Gene Id114215
Protein NameQ9WUK0
Description of TargetInsulin-like 3
Uniprot IDhttps://www.uniprot.org/uniprot/Q9WUK0
Protein Accession #NP_446132.1
Nucleotide Accession #NM_053680.1
Protein Size (# AA)128
Molecular Weight14 kDa
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