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Catalog No: OKEH00291
Size:96T
Price: $525.00
SKU
OKEH00291
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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INSL3 ELISA Kit (Human) : 96 Wells (OKEH00291)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology INSL3 ELISA Kit (Human) (OKEH00291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for INSL3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for INSL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample INSL3 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
INSL3 Microplate96 Wells (12 x 8 Well strips)
INSL3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated INSL3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity7.9 pg/mL
SpecificityNatural and recombinant Human Insulin-like 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolINSL3
Gene Full Nameinsulin like 3
Alias Symbolsinsulin-like 3;insulin-like 3 (Leydig cell);leydig insulin -like hormone;leydig insulin-like peptide;ley-I-L;prepro-INSL3;Relaxin-like factor;relaxin-like factor b;RLF;RLNL.
NCBI Gene Id3640
Protein NameP51460
Description of TargetInsulin-like 3
Uniprot IDhttps://www.uniprot.org/uniprot/P51460
Protein Accession #NP_001252516.1
Nucleotide Accession #NM_001265587.1
Protein Size (# AA)131
Molecular Weight15 kDa
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