INS ELISA Kit (Human) : 96 Wells (OKEH00456)

Catalog No: OKEH00456

Size:96T

Human INS ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology INS ELISA Kit (Human) (OKEH00456) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human INS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated INS and incubated. The INS found in the sample or standards competes with the biotinylated INS for limited binding sites on the immobilized anti-Human INS antibody. Excess unbound biotinylated INS and sample or standard INS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated INS captured in the well and inversely proportional to the amount of INS which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100 mIU/L
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.8% (n = 20)
  Mean Inter-assay CV%: < 9.2% (n = 20)
• ELISA Kit Component:
  • Anti-INS Microplate: 96 Wells (12 x 8 Well strips)
  • INS Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X INS-Biotn Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.78mIU/L
• Specificity: Natural and recombinant Human Insulin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: INS
• Gene Full Name: insulin
• Alias Symbols: IDDM;IDDM1;IDDM2;ILPR;insulin;IRDN;MODY10;PNDM4;preproinsulin;proinsulin.
• NCBI Gene Id: 3630
• Protein Name: P01308
• Description of Target: Insulin
• Uniprot ID: https://www.uniprot.org/uniprot/P01308
• Protein Accession #: NP_000198.1
• Nucleotide Accession #: NM_000207.2
• Protein Size (# AA): 110
• Molecular Weight: 11 kDa