INPP5D Antibody (OAAF00916)

Catalog No: OAAF00916 (Formerly GWB-ASC329)

Size:100 ug

Western blot analysis of extracts from mouse brain cells, using SHIP1 Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human SHIP1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: SHIP1 (Ab-1020) antibody detects endogenous levels of total SHIP1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:5000
• Reference: Rossana Trotta, Blood, Apr 2005; 105: 3011 - 3018.
  Jacqueline M. Mason, J. Biol. Chem., Feb 2000; 275: 4398.
  Christopher J Ong, Blood, May 2007; 10.1182/blood-2007-03-079699.
  Huazhang An, Hongmei Xu, Blood, Jun 2005; 105: 4685 - 4692.

Target Info

• Gene Symbol: INPP5D
• Gene Full Name: inositol polyphosphate-5-phosphatase D
• Alias Symbols: hp51CN;Inositol polyphosphate-5-phosphatase of 145 kDa;inositol polyphosphate-5-phosphatase, 145kD;inositol polyphosphate-5-phosphatase, 145kDa;p150Ship;phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1;phosphatidylinositol 4,5-bisphosphate 5-phosphatase;SH2 domain-containing inositol 5'-phosphatase 1;SHIP;SHIP1;SHIP-1;signaling inositol polyphosphate 5 phosphatase SIP-145;signaling inositol polyphosphate phosphatase SHIP II;SIP-145.
• NCBI Gene Id: 3635
• Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• Description of Target: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
• Uniprot ID: Q92835
• Protein Accession #: NP_001017915.1
• Molecular Weight: 133 kDa