INPP5B Antibody (OAGA00969)

Catalog No: OAGA00969

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
OAGA00969 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using INPP5B(OAGA00969) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). This gene encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
  Positive Controls: H1299, HeLa, HepG2, Molt-4, Raji
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 699 and 949 of INPP5B (Uniprot ID#P32019)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.34 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: INPP5B
• Gene Full Name: Type II inositol 1,4,5-trisphosphate 5-phosphatase
• Alias Symbols: 5PTase
• NCBI Gene Id: 3633
• Protein Name: Type II inositol 1,4,5-trisphosphate 5-phosphatase
• Uniprot ID: P32019
• Protein Accession #: NP_005531.2
• Molecular Weight: 104 kDa