Catalog No: OKCD01221 (Formerly GWB-KBBRW2)
Size:96WELLS
Price: $750.00
SKU
OKCD01221
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for INPP4A ELISA Kit (Human) (OKCD01221) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 30 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A). No significant cross-reactivity or interference between Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | INPP4A |
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Gene Full Name | inositol polyphosphate-4-phosphatase type I A |
Alias Symbols | Inositol polyphosphate 4-phosphatase type I;inositol polyphosphate-4-phosphatase type I A;inositol polyphosphate-4-phosphatase, type I, 107kD;inositol polyphosphate-4-phosphatase, type I, 107kDa;INPP4;TVAS1;type I inositol 3,4-bisphosphate 4-phosphatase. |
NCBI Gene Id | 3631 |
Protein Name | Type I inositol 3,4-bisphosphate 4-phosphatase |
Description of Target | Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate. |
Uniprot ID | Q96PE3 |
Protein Accession # | NP_001127696 |
Nucleotide Accession # | NM_001134224.1 |
Protein Size (# AA) | 977 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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