Catalog No: OKCD01221 (Formerly GWB-KBBRW2)
Size:96WELLS
Price: $750.00
SKU
OKCD01221
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Datasheets/ManualsPrintable datasheet for INPP4A ELISA Kit (Human) (OKCD01221)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-INPP4A Microplate96 Wells (12 x 8 Well Strips)
INPP4A Lyophilized Standard2 x 5 ng
100X Biotinylated INPP4A Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 30 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A). No significant cross-reactivity or interference between Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolINPP4A
Gene Full Nameinositol polyphosphate-4-phosphatase type I A
Alias SymbolsInositol polyphosphate 4-phosphatase type I;inositol polyphosphate-4-phosphatase type I A;inositol polyphosphate-4-phosphatase, type I, 107kD;inositol polyphosphate-4-phosphatase, type I, 107kDa;INPP4;TVAS1;type I inositol 3,4-bisphosphate 4-phosphatase.
NCBI Gene Id3631
Protein NameType I inositol 3,4-bisphosphate 4-phosphatase
Description of TargetCatalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate.
Uniprot IDQ96PE3
Protein Accession #NP_001127696
Nucleotide Accession #NM_001134224.1
Protein Size (# AA)977
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