Inositol Triphosphate ELISA Kit (OKCD02270)

Catalog No: OKCD02270

Size:96WELLS

IP3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	85-99%	94-101%	89-103%	85-104%
  EDTA plasma(n=5)	78-99%	87-98%	96-103%	79-104%
  heparin plasma(n=5)	83-105%	78-99%	83-99%	91-98%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inositol Triphosphate (IP3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inositol Triphosphate (IP3) and unlabeled Inositol Triphosphate (IP3) (Standards or samples) with the pre-coated antibody specific to Inositol Triphosphate (IP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample.
• ELISA Kit Range: 123.5-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	78-94	84
  EDTA plasma(n=5)	81-91	88
  heparin plasma(n=5)	85-103	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Inositol Triphosphate Microplate: 96 Wells (12 x 8 Well Strips)
  • Inositol Triphosphate Lyophilized Standard: 2
  • 100X Inositol Triphosphate Biotin Complex: 120 uL
  • 100X Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Biotin Complex Diluent: 1 x 12 mL
  • Avidin/HRP Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Additional Information: CAS: 85166-31-0
  PubChem: 439456
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 49.3 pg/mL
• Assay Info: Assay Methodology: Competitive Inhibition Immunoassay

Target Info

• Gene Symbol: IP3
• Alias Symbols: Inositol 1,4,5-trisphosphate; 1,4,5-Insp3; D-myo-Inositol 1,4,5-trisphosphate; InsP3; 1D-myo-Inositol 1,4,5-trisphosphate; D-myo-inositol-1,4,5-triphosphate
• Protein Name: Inositol Triphosphate
• Description of Target: Intracellular messenger formed by the action of phospholipase C on phosphatidylinositol 4,5-bisphosphate, which is one of the phospholipids that make up the cell membrane. Inositol 1,4,5-trisphosphate is released into the cytoplasm where it releases calcium ions from internal stores within the cell's endoplasmic reticulum. These calcium ions stimulate the activity of B kinase or calmodulin.