INHA ELISA Kit (Pig) (OKCD02645)

Catalog No: OKCD02645

Size:96WELLS

INHA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-101%	97-105%	79-101%	88-98%
  EDTA Plasma (n=5)	83-99%	90-97%	80-104%	80-99%
  Heparin Plasma (n=5)	78-99%	98-105%	89-97%	90-97%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inhibin Alpha (INHa) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inhibin Alpha (INHa) and unlabeled Inhibin Alpha (INHa) (Standards or samples) with the pre-coated antibody specific to Inhibin Alpha (INHa). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin Alpha (INHa) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inhibin Alpha (INHa) in the sample.
• ELISA Kit Range: 12.35-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-99	87
  EDTA Plasma (n=5)	81-103	95
  Heparin Plasma (n=5)	87-96	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibin Alpha (INHa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibin Alpha (INHa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-INHA Microplate: 96 Wells (12 x 8 Well strips)
  • INHA Lyophilized Standard: 2 x 1 ng
  • 100X INHA Biotin Complex: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 5.16 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Inhibin A (INHA). No significant cross-reactivity or interference between Inhibin A (INHA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: INHA
• Gene Full Name: inhibin subunit alpha
• Alias Symbols: inhibin alpha chain;inhibin alpha subunit.
• NCBI Gene Id: 397386
• Protein Name: Inhibin alpha chain
• Description of Target: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
• Uniprot ID: P04087
• Protein Accession #: NP_999354.1
• Nucleotide Accession #: NM_214189.1
• Molecular Weight: 364 kDa