IL7R/CD127 ELISA Kit (Mouse) (OKBB00430)

Catalog No: OKBB00430

Size:96 Wells

Mouse IL7R/CD127 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL7R has been precoated onto 96-well plates. Standards(NS0, E21-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse IL7R amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	2	6.5	12.8	2.3	7.3	14.5
  SD	0.114	0.442	0.576	0.145	0.555	0.725
  CV%	5.7	6.8	4.5	6.3	7.6	5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse IL7R Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse IL7R Standard: 2 x 20 ng/tube
  • 100X Biotinylated Anti-Mouse IL7R Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants and serum
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse IL7R
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E21-D239
• Description: For quantitative detection of mouse IL7R in cell culture supernatants and serum.

Target Info

• Gene Symbol: IL7R
• Gene Full Name: interleukin 7 receptor
• Alias Symbols: CD 127, CD127, CD127 antigen, CDw127, IL 7R alpha, IL 7R, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, IL7RA, IL7RA_HUMAN, IL7Ralpha, ILRA, Interleukin 7 receptor alpha chain, Interleukin 7 receptor, Interleukin 7 receptor isoform H5 6, Interleukin-7 receptor subunit alpha
• NCBI Gene Id: 16197
• Protein Name: Interleukin-7 receptor subunit alpha
• Description of Target: The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
• Uniprot ID: P16872
• Protein Accession #: NP_032398.3
• Nucleotide Accession #: NM_008372.4