IL5RA ELISA Kit (Human) : 96 Wells (OKEH00282)

Catalog No: OKEH00282

Size:96T

Human IL5RA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IL5RA ELISA Kit (Human) (OKEH00282) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL5RA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for IL5RA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL5RA captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.7% (n = 20)
  Mean Inter-assay CV%: < 9.7% (n = 20)
• ELISA Kit Component:
  • IL5RA Microplate: 96 Wells (12 x 8 Well strips)
  • IL5RA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated IL5RA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 32 pg/mL
• Specificity: Natural and recombinant Human Interleukin-5 receptor subunit alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL5RA
• Gene Full Name: interleukin 5 receptor subunit alpha
• Alias Symbols: CD125;CD125 antigen;CDw125;HSIL5R3;IL-5 receptor subunit alpha;IL5R;IL-5R subunit alpha;interleukin 5 receptor type 3;interleukin 5 receptor, alpha;interleukin-5 receptor alpha chain;interleukin-5 receptor subunit alpha.
• NCBI Gene Id: 3568
• Protein Name: Q01344
• Description of Target: Interleukin-5 receptor subunit alpha
• Uniprot ID: https://www.uniprot.org/uniprot/Q01344
• Protein Accession #: NP_000555.2
• Nucleotide Accession #: NM_000564.4
• Protein Size (# AA): 420
• Molecular Weight: 48 kDa