IL36G ELISA Kit (Mouse) (OKEH03002)

Catalog No: OKEH03002 (Formerly GWB-7E2TNG)

Size:96T

Mouse IL36G ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IL36G ELISA Kit (Mouse) (OKEH03002) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il36g has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Il36g is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il36g captured in well.
• ELISA Kit Range: 15.6-1000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <6.5 % (n = 20)
  Mean Inter-assay CV%: < 9.1% (n = 20)
• ELISA Kit Component:
  • Anti-Il36g Microplate: 96 Wells (12 x 8 Well strips)
  • Il36g Lyophilized Standard: 2
  • 100X Biotinylated Il36g Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 7.88 pg/mL
• Specificity: Natural and recombinant Mouse Interleukin-36 gamma
• Publications:

  Koss, Carolin K et al. IL36 is a critical upstream amplifier of neutrophilic lung inflammation in mice. Commun Biol. 2021 4: 172. 33558616

  IL36 is a critical upstream amplifier of neutrophilic lung inflammation in mice. Commun Biol. 4, 172 (2021). 34808192

  Tbet and IL-36γ cooperate in therapeutic DC-mediated promotion of ectopic lymphoid organogenesis in the tumor microenvironment. Oncoimmunology. 6, e1322238 (2017). 28680760

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for IL36G ELISA Kit (Mouse) (OKEH03002) on CiteAb

Target Info

• Gene Symbol: IL36G
• Gene Full Name: interleukin 1 family, member 9
• Alias Symbols: Interleukin-1 family member 9.
• NCBI Gene Id: 215257
• Protein Name: Q8R460
• Description of Target: Interleukin-36 gamma
• Uniprot ID: https://www.uniprot.org/uniprot/Q8R460
• Protein Accession #: NP_705731.2 
• Nucleotide Accession #: NM_153511.3
• Protein Size (# AA): 164
• Molecular Weight: 19 kDa