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Catalog No: OKCD00209 (Formerly GWB-KBBSH4)
Size:96WELLS
Price: $750.00
SKU
OKCD00209
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Datasheets/ManualsPrintable datasheet for IL36G ELISA Kit (Human) (OKCD00209)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)95-103%80-105%85-101%82-103%
EDTA plasma(n=5)95-105%90-98%82-98%79-95%
heparin plasma(n=5)96-103%96-103%96-105%94-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Family, Member 9 (IL1F9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Family, Member 9 (IL1F9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Family, Member 9 (IL1F9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Family, Member 9 (IL1F9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-9692
EDTA plasma(n=5)94-10398
heparin plasma(n=5)97-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Family, Member 9 (IL1F9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Family, Member 9 (IL1F9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IL36G Microplate96 Wells (12 x 8 Well Strips)
IL36G Lyophilized Standard2 x 1 ng
100X Biotinylated IL36G Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 5.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Family, Member 9 (IL1F9). No significant cross-reactivity or interference between Interleukin 1 Family, Member 9 (IL1F9) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL36G
Gene Full Nameinterleukin 36 gamma
Alias SymbolsIL-1 related protein 2;IL1E;IL-1-epsilon;IL1F9;IL-1F9;IL1H1;IL-1H1;IL-1-related protein 2;IL1RP2;IL-1RP2;interleukin 1-related protein 2;interleukin-1 epsilon;interleukin-1 family member 9;interleukin-1 homolog 1;interleukin-36 gamma.
NCBI Gene Id56300
Protein NameInterleukin-36 gamma
Description of TargetCytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
Uniprot IDQ9NZH8
Protein Accession #NP_001265497
Nucleotide Accession #NM_001278568.1
Protein Size (# AA)169
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