Catalog No: OKAA00021-96W
Size:96 Wells
Price: $450.00
SKU
OKAA00021-96W
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||
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Application | ELISA | ||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||
ELISA Kit Reproducibility | The coefficient of variation of both intra-assay and inter-assay were less than 10%. | ||||||||||||
ELISA Kit Component |
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Additional Information | Sample Type: cell culture supernates, serum, and plasma | ||||||||||||
:: | Reagents: REAGENTS 1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-32?(8x12) 2. 2 vials human IL-32? Standard lyophilized, 500 pg/ml upon reconstitution 3. 2 vials concentrated Biotin-Conjugate anti-human IL-32? monoclonal antibody 4. 2 vials Streptavidin-HRP solution, 5. 1 bottle Standard /sample Diluent 6. 1 bottle Biotin-Conjugate antibody Diluent 7. 1 bottle Streptavidin-HRP Diluent 8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 9. 1 vial Substrate Solution 10. 1 vial Stop Solution 11. 3 pieces Adhesive Films 12. package insert | ||||||||||||
Sample Type | cell culture, supernates, serum, and plasma | ||||||||||||
Sensitivity | 7 pg/ml | ||||||||||||
Predicted Homology Based on Immunogen Sequence | Human | ||||||||||||
Specificity | This assay recognizes both natural and recombinant human IL-22. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed. Recombinant human: IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6 , Recombinant mouse: IL-1a, IL-1b, IL-2, IL-5, IL-6, IL-7 , Recombinant rat: IL-2, IL-4, IL-6, IL-10, IL-22 | ||||||||||||
Assay Info | Assay Method: Quantitative Sandwich ELISA |
Gene Symbol | IL32 |
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Gene Full Name | interleukin 32 |
Alias Symbols | NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd, IL-32beta, IL-32alpha, IL-32delta, IL-32gamma |
NCBI Gene Id | 9235 |
Protein Name | Interleukin-32 |
Description of Target | This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Uniprot ID | P24001 |
Protein Accession # | NP_001012649.1 |
Nucleotide Accession # | NM_001012631.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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