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IL25 ELISA Kit (Mouse) : 48 Wells (OKAA00040_48W)

  • Catalog#: OKAA00040_48W
  • 2 weeks

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Free trial-size samples may be available for this item. Please go here for more information.
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48 Wells
$185.00
In Stock
Free trial-size samples may be available for this item. Please go here for more information.
Application:
ELISA
Gene Symbol:
IL25
Official Gene Full Name:
interleukin 25
NCBI Gene Id:
140806
Alias Symbols:
Il17e, IL-17e
Swissprot Id:
Q8VHH8
Protein Accession #:
NP_542767.1
Nucleotide Accession #:
NM_080729.3
Species Reactivity:
Mouse
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
15 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials mouse IL-25/IL-17E Standard lyophilized, 1000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-mouse IL-25/IL-17E antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL25.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL25.
Predicted Homology Based on Immunogen Sequence:
Mouse
Datasheets / Downloads:
OKAA00040_48W
Specificity:
This assay recognizes both natural and recombinant mouse IL-2p70. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.

Recombinant human: IL-6, IL-6sR, IL-12p35, IL-12p40 monomer, IL-12p70

Recombinant mouse: IL-10, IL-12p35, IL-12p40 monomer, TNF-a

Recombinant porcine:
Assay Info:
Assay Method: Quantitative Sandwich ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma.
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with antibody to mouse IL-25/IL-17E (8x12)
2. 2 vials mouse IL-25/IL-17E Standard lyophilized, 1000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-mouse IL-25/IL-17E antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Protein Name:
interleukin-25
Protocol:
Tips Information:
 
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