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- Official Gene Full Name:
- interleukin 25
- NCBI Gene Id:
- Alias Symbols:
- Il17e, IL-17e, IL-17E
- Protein Name:
- Species Reactivity:
- Sample Type:
- cell culture, supernates, serum, and plasma
- 15 pg/ml
- Kit Reproducibility:
- The coefficient of variation of both intra-assay and inter-assay were less than 10%.
- Kit Component:
|2 vials mouse IL-25/IL-17E Standard lyophilized, 1000 pg/ml upon reconstitution|
|2 vials concentrated Biotin-Conjugate anti-mouse IL-25/IL-17E antibody|
|2 vials Streptavidin-HRP solution, |
|1 bottle Standard /sample Diluent|
|1 bottle Biotin-Conjugate antibody Diluent |
|1 bottle Streptavidin-HRP Diluent |
|1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)|
|1 vial Substrate Solution |
|1 vial Stop Solution|
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express IL25.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express IL25.
- Predicted Homology Based on Immunogen Sequence:
- This assay recognizes both natural and recombinant mouse IL-2p70. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IL-6, IL-6sR, IL-12p35, IL-12p40 monomer, IL-12p70
Recombinant mouse: IL-10, IL-12p35, IL-12p40 monomer, TNF-a
- Assay Info:
- Assay Method: Quantitative Sandwich ELISA
- Additional Information:
- Sample Type: cell culture supernates, serum, and plasma.
- Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with antibody to mouse IL-25/IL-17E (8x12)
2. 2 vials mouse IL-25/IL-17E Standard lyophilized, 1000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-mouse IL-25/IL-17E antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
- Tips Information: