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Catalog No: OKCD00444
Size:96 Wells
Price: $659.00
SKU
OKCD00444
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IL22RA1 ELISA Kit (Human) (OKCD00444)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
83-101%91-102%93-104%78-94%
ELISA Kit PrincipleAviva Systems Biology IL22RA1 ELISA Kit (Human) (OKCD00444) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL22RA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL22RA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL22RA1captured in well.
ELISA Kit Range0.156-10 ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
80-10289
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level IL22RA1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level IL22RA1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-IL22RA1 Microplate96 Wells (12 x 8 Well Strips)
IL22RA1 Lyophilized Standard2 x 10 ng
100X Biotinylated IL22RA1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 22 Receptor (IL22R). No significant cross-reactivity or interference between Interleukin 22 Receptor (IL22R) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL22RA1
Gene Full Nameinterleukin 22 receptor subunit alpha 1
Alias SymbolsIL22R, CRF2-9, IL22R1
NCBI Gene Id58985
Protein Nameinterleukin-22 receptor subunit alpha-1
Description of TargetComponent of the receptor for IL20, IL22 and IL24. Component of IL22 receptor formed by IL22RA1 and IL10RB enabling IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. Mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation.6 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.6"Identification of the functional interleukin-22 (IL-22) receptor complex: the IL-10R2 chain (IL-10Rbeta) is a common chain of both the IL-10 and IL-22 (IL-10-related T cell-derived inducible factor, IL-TIF) receptor complexes." Kotenko S.V., Izotova L.S., Mirochnitchenko O.V., Esterova E., Dickensheets H., Donnelly R.P., Pestka S. J. Biol. Chem. 276:2725-2732(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBUNIT.Ref.7"STAT activation by IL-19, IL-20 and mda-7 through IL-20 receptor complexes of two types." Dumoutier L., Leemans C., Lejeune D., Kotenko S.V., Renauld J.-C. J. Immunol. 167:3545-3549(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.9"Interleukin 24 (MDA-7/MOB-5) signals through two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2." Wang M., Tan Z., Zhang R., Kotenko S.V., Liang P. J. Biol. Chem. 277:7341-7347(2002) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.10"Interleukins 19, 20, and 24 signal through two distinct receptor complexes. Differences in receptor-ligand interactions mediate unique biological functions." Parrish-Novak J., Xu W., Brender T., Yao L., Jones C., West J., Brandt C., Jelinek L., Madden K., McKernan P.A., Foster D.C., Jaspers S., Chandrasekher Y.A. J. Biol. Chem. 277:47517-47523(2002) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.11"Melanoma differentiation-associated gene 7/interleukin (IL)-24 is a novel ligand that regulates angiogenesis via the IL-22 receptor." Ramesh R., Mhashilkar A.M., Tanaka F., Saito Y., Branch C.D., Sieger K., Mumm J.B., Stewart A.L., Boquoi A., Dumoutier L., Grimm E.A., Renauld J.-C., Kotenko S., Chada S. Cancer Res. 63:5105-5113(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.14"IL-22-mediated liver cell regeneration is abrogated by SOCS-1/3 overexpression in vitro." Brand S., Dambacher J., Beigel F., Zitzmann K., Heeg M.H.J., Weiss T.S., Pruefer T., Olszak T., Steib C.J., Storr M., Goeke B., Diepolder H., Bilzer M., Thasler W.E., Auernhammer C.J. Am. J. Physiol. 292:G1019-G1028(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.
Uniprot IDQ8N6P7
Protein Accession #NP_067081
Nucleotide Accession #NM_021258.3
Protein Size (# AA)574
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